Biodiagnosis of Soil with a Low Level of Anthropogenic Impact (with the typical Urban Settlement Stepnoe, Saratov Region, as an example)
With the urban-type settlement Stepnoe as an example, biodiagnosis of the soil of a settlement with low technogenic impact was conducted. In the course of our work, 30 soil samples were plated onto solid nutrition media to evaluate the following: the total numbers of heterotrophic microorganisms - on meat peptone agar, the numbers of hydrocarbon-oxidizing and iron-oxidizing microorganisms - on their corresponding selective media, and the activity of several soil redox and hydrolytic enzymes (dehydro-genases, catalases, peroxidases, and invertases) were also studied. To characterize the degree of anthropogenic impact on the soil, the content of the mobile forms of copper, zinc, lead, cadmium, chromium and nickel was evaluated and the total soil contamination coefficient (Zc) was calculated. Within the studied area, an excess over the MPC of the mobile forms of Ni, Cu and Pb was found. In general, the values of the total soil pollution coefficient did not exceed 16 units, indicating a favorable environmental and geochemical situation in the territory of the settlement. Analysis of the total numbers of heterotrophic microorganisms, numbers of hydrocarbon-oxidizing and iron-oxidizing microorganisms, revealed deviations from the norm in certain regions of the territory surveyed, indicating an anthropogenic transformation of the soil biocenoses. The maximum deviations were typical for the index of hydrocarbon-oxidizing microorgan-isms, which indicated soil contamination with hydrocarbons. Our results of evaluation of the activity of indicator soil enzymes did not exclude possible functional damages in the soil. It was found that the activity of soil dehydrogenases and invertases corresponded to low and very low levels of soil enrichment with these enzymes. At the same time, the activity of soil catalases and peroxidases corresponded to the average and high levels of these enzymes, which indicated the absence of pathological changes in the soil. It has been shown that the studied soil microbiological and biochemical parameters are promising for soil health biodiagnosis in territories with low anthropogenic impact.
Alekseenko V. A., Alekseenko A. V. Chemical Elements in Geochemical Systems. The Elements Abundances in Urban Soils. Rostov-on-Don, Izdatelstvo Yuzhnogo federalnogo universiteta, 2013. 380 p. (in Russian).
Galiulin R. V., Galiulina R. A. Enzymatic indication of soil contamination with heavy met-als. Agrochemistry, 2006, no. 11, pp. 84–95 (in Russian).
GOST 17.4.4.02-84. Okhrana prirody. Pochvy. Metody otbora i podgotovki prob dlya khimicheskogo, bakteriologicheskogo, gelmintologicheskogo analiza: Mezhgosudarstvennyye standarty [GOST 17.4.4.02-84. Protection of Nature. The soil. Methods of sampling and sample preparation for chemical, bacteriological, helminthological analysis: Interstate standards]. Mos-cow, Standardinform Publ., 2008. 8 p. (in Russian).
Evdokimova G. A., Mozgova N. R. The impact of emissions from the nonferrous metallurgical plant on soil in a model experiment. Eurasian Soil Science, 2000, vol. 33, no. 5, pp. 552–559.
Zvyagintsev D. G. Soil biological activity and scales for evaluating some of its indicators. Pochvovedeniye, 1978, no. 6, pp. 48–54 (in Russian).
Ivanov D. V. Heavy metals in soils of the Republic of Tatarstan (an overview). Russian J. of Applied Ecology, 2015, no. 4, pp. 53–50 (in Russian).
Ismailov N. M. Microbiological and enzymatic activity of oil-polluted soils. In: M. A. Glazovskaya, ed. Restoration of oil-polluted soil ecosystems. Moscow, Nauka Publ., 1988, pp. 42–56 (in Russian).
Kazeev K. Sh., Kolesnikov S. I., Valkov V. F. Biological diagnostics and indication of soils: methodology and methods of researches. Rostov-on-Don, Izdatelstvo Rostovskogo universiteta, 2003. 204 p. (in Russian).
Kvesitadze G. I., Khatisashvili G. A., Sadunishvili T. A., Evstigneeva Z. G. Metabolizm antropogennykh toksikantov v vysshikh rasteniyakh [Metabolism of anthropogenic toxicants in higher plants]. Moscow, Nauka Publ., 2005. 199 p. (in Russian).
Kireeva N. A., Novoselova E. I., Onegova T. S. Catalase and dehydrogenase activity in soils polluted by oil and oil products. Agrochemistry, 2002, no. 8, pp. 64–72 (in Russian).
Kudryashov S. V. Ocenka i normirovanie ehkologicheskogo sostoyaniya pochv Norilskogo promyshlennogo rajona [Assessment and Regulation of the Ecological State of the Soils of the Norilsk Industrial Region]. Thesis Diss. Cand. Sci. (Biol.). Moscow, 2010. 17 p. (in Russian).
Lapina G. P., Chernavskaya N. M., Litvinovsky M. E., Sazanova S. V. Physico-chemical characteristics of environmental pollution in man-made disasters (oil spills). Chemical and Biological Safety, 2007, no. 1, vol. 31, pp. 24–32 (in Russian).
Guidelines for the determination of heavy metals in soils of farmland and plant products. Moscow, CINAO, 1992. 62 p. (in Russian).
Mynbayeva B. N., Seylova L. B., Voronova N. V., Muzdybaeva K. K., Amirasheva B.A., Imanbekova T. G. Microbiological indication of soils contaminated with heavy me tals in Almaty. Reports on Ecological Soil Science, 2013, no. 1, pp. 176–184 (in Russian).
Panov A. V., Esikova T. Z., Sokolov S. L., Kosheleva I. A., Boronin A. M. Influence of soil pollution on the composition of a microbial community. Microbiology, 2013, vol. 82, no. 2, pp. 241–248. DOI: 10.7868/S0026365613010114
Pleshakova E. V., Reshetnikov M. V., Ngun K. T., Shuvalova E. P. Microbiological and biochemical indication of the soil of the city of Mednogorsk. Agrochemistry, 2016, no. 1, pp. 66–73 (in Russian).
Workshop in Microbiology. A. I. Netrusov, ed. Moscow, Akademiya Publ., 2005. 608 p. (in Russian).
Regions and cities of Russia: an integral assessment of the ecological state. Ed. N. S. Kasimov. Moscow, Molodaya gvardiya Publ., 2015. 661 p. (in Russian).
Smagin A. V., Shoba S. A., Makarov O. A. Ekologicheskaya otsenka pochvennykh resursov i tekhnologii ikh vosproizvodstva (na primere g. Moskvy) [Ecological assessment of soil resources and technologies of their reproduction (on the example of Moscow)]. Moscow, Izdatelstvo Mosk-ovskogo universiteta, 2008. 360 p. (in Russian).
Sorokin N. D., Grodnitskaya I. D., Shapchenkova O. A., Evgrafova S. Yu. Experimental assessment of the microbocenosis stability in chemically polluted soils. Eurasian Soil Science, 2009, vol. 42, no. 6, pp. 650–656.
Khaziev F. Kh. Methods of soil enzymology. Moscow, Nauka Publ., 2005. 252 p. (in Russian).
Das S. K., Varma A. Role of enzymes in maintaining soil health. In: G. Shukla, A. Varma, eds. Soil Enzymology (Soil Biology 22). Berlin, Heidelberg, Springer-Verlag, 2011, pp. 25–41. DOI: 10.1007/978-3-642-14225-3_2
Fliepbach A., Martens R., Reber H. Soil microbial biomass and activity in soils treated with heavy metal contaminated sewage sludge. Soil Biology and Biochemistry, 1994, vol. 26, pp. 1201–1205.
Floch C., Alarcon-Gutierrez E., Criquet S. ABTS assay of phenol oxidase activity in soil. J. of Microbiological Methods, 2007, vol. 71, pp. 319–324.
Foght J., Aislabie J. Enumeration of soil microorganisms. In: R. Margesin, F. Schinner, eds. Manual for soil analysis – monitoring and assessing soil bioremediation. Berlin, Heidelberg, Springer-Verlag, 2005, pp. 261–280.
Franzaring J., Hrenn H., Schumm C., Klumpp A., Fangmeier A. Environmental monitoring of fluoride emiddion using precipitation, dust, plant and soil samples. Environmental Рollution, 2006, vol. 1, pp. 158–165.
García C., Hernández T. Biological and biochemical indicators in derelict soils subjected to erosion. Soil Biology and Biochemistry, 1997, vol. 29, pp. 171–177.
Gennadiev A. N., Pikovskii Yu. I., Tsibart A. S., Smirnova M. A. Hydrocarbons in soils: origin, composition, and behavior (Review). Eurasian Soil Science, 2015, vol. 48, no. 10, pp. 1076–1089. DOI: 10.1134/S1064229315100026
Gianfreda L., Mora M. L., Diez M. C. Restoration of polluted soils by means of microbial and enzymatic рrocesses. Revista de la ciencia del suelo y nutrición vegetal, 2006, vol. 6, no. 1, pp. 20–40.
Granina L. Z., Parfenova V. V., Zemskaya T. I., Zakharova Yu. R., Golobokova L. P. On iron and manganese oxidizing microorganisms in sedimentary redox cycling in lake Baikal. Berliner Palaobiologische Abhandlungen, 2003, vol. 4, pp. 121–128.
Guo H., Yao J., Cai M., Qian Y., Guo Y., Richnow H. H., Blake R. E., Doni S., Ceccanti B. Effects of petroleum contamination on soil microbial numbers, metabolic activity and urease activity. Chemosphere, 2012, vol. 87, pp. 1273–1280. DOI: 10.1016/j.chemosphere.2012.01.034
Hassan W., Akmal M., Muhammad I., Younas M., Zahaid K. R., Ali F. Response of soil mi-crobial biomass and enzymes activity to cadmium (Cd) toxicity under different soil textures and incubation times. Australian J. of Crop Science, 2013, vol. 7, no. 5, pp. 674–680.
Kızılkaya R., Aşkın T., Bayrakli B., Sağlam M. Microbiological characteristics of soils contaminated with heavy metals. European J. of Soil Biology, 2004, vol. 40, no. 2, pp. 95–102. DOI: 10.1016/j.ejsobi.2004.10.002
Kraus J. J., Munir I. Z., McEldoon J. P., Clark D. S., Dordick J. S. Oxidation of polycyclic aromatic hydrocarbons catalyzed by soybean peroxidase. Applied Biochemistry and Biotechnology, 1999, vol. 80, no. 3, pp. 221–230.
Maila M. P., Cloete T. E. The use of biological activities to monitor the removal of fuel con-taminants – perspective for monitoring hydrocarbon contamination: a review. International Biodeterioration and Biodegradation, 2005, vol. 55, pp. 1–8. DOI:10.1016/j.ibiod.2004.10.003
Margesin R., Zimmerbauer A., Schinner F. Monitoring of bioremediation by soil biological activities. Chemosphere, 2000 a, vol. 40, no. 4, pp. 339–346.
Margesin R., Walder G., Schinner F. The impact of hydrocarbon remediation (diesel oil and polycyclic aromatic hydrocarbons) on enzyme activities and microbial properties of soil. Acta Biotechnologica, 2000 b, vol. 20, pp. 313–333.
Mills A., Breuil LuC., Colwell R. R. Enumeration of petroleum degrading marine and estua-rine microorganisms by the most probable number method. Canadian J. of Microbiology, 1978, vol. 24, pp. 552–557.
Murata T., Kanao-Koshikawa M., Takamatsu T. Effects of Pb, Cu, Sb, Zn and Ag contami-nation on the proliferation of soil bacterial colonies, soil dehydrogenase activity, and phospholipid fatty acid profiles of soil microbial communities. Water, Air, and Soil Pollution, 2005, vol. 164, pp. 103–118.
Muratova A., Pozdnyakova N., Golubev S., Wittenmayer L., Makarov O., Merbach W., Turkovskaya O. Oxidoreductase activity of sorghum root exudates in a phenanthrene-contaminated environment. Chemosphere, 2009, vol. 74, pp. 1031–1036.
Ofoegbu C. J., Akubugwo E. I., Dike C. C., Maduka H. C. C., Ugwu C. E., Obasi N. A. Effects of heavy metals on soil enzymatic activities in the Ishiagu mining area of Ebonyi State-Nigeria. J. of Environmental Science, Toxicology and Food Technology, 2013, vol. 5, no. 6, pp. 66–71.
Öhlinger R. Dehydrogenase activity with the substrate TTC. In: F. Schinner, R. Ohlinger, E. Kandler, R. Margesin, eds. Methods in Soil Biology. Berlin, Springer Verlag, 1996, pp. 241–243.
Rylovа N. G., Stepus N. F. The change of cellulose soil activity as a result of pollution with heavy metals. Biology, 2005, vol. 10, pp. 65–69.
Sabrina H., Michael S., Johnson B. The iron-oxidizing proteobacteria. Microbiology, 2011, vol. 157, pp. 1551–1564.
Salah E. A. M., Yassin K. H., Abd-Alsalaam S. Level, distribution and pollution assessment of heavy metals in urban community garden soils in Baghdad City, Iraq. International J. of Scientific Engineering and Research, 2015, vol. 6, no. 10, pp. 1646–1652.
Shi Z. J., Lu Y., Xu Z. G., Fu S. L. Enzyme activities of urban soils under different land use in the Shenzhen city, China. Plant Soil and Environment, 2008, vol. 54, iss. 8, pp. 341–346.
Shuqing L., Zhixin Y., Xiaomin W., Xiaogui Z., Rutai G., Xia L. Effects of Cd and Pb pollution on soil enzymatic activities and soil microbiota. Frontiers of Agriculture in China, 2007, vol. 1, iss. 1, pp. 85–89.
Su C., Jiang L., Zhang W. A. review on heavy metal contamination in the soil worldwide: Situation, impact and remediation techniques. Environmental Skeptics and Critics, 2014, vol. 3, no. 2, pp. 24–38.
Sumampouw O. J., Risjani Y. Bacteria as indicators of environmental pollution: Review. International J. of Ecosystems and Ecology Science, 2014, vol. 4, no. 6, pp. 251–258. DOI: 10.5923/j.ije.20140406.03
Tuomela M., Steffen K., Kerko E. Influence of Pb contamination in boreal forest soil on the growth and ligninolytic activity of litter-decomposing fungi. Microbiology Ecology, 2005, vol. 53, pp. 179–1862.
Utobo E. B., Tewari L. Soil enzymes as bioindicators of soil ecosystem status. Applied Ecology and Environmental Research, 2015, vol. 13, no. 1, pp. 147–169. DOI: 10.15666/aeer/1301_147169
Wilkinson S. Nicklin S., Faul J. L. Biodegradation of fuel oils and lubricants: soil and water bioremediation options. In: V. P. Singh, R. D. Stapleton, eds. Bionransformations: bioremediation technology for health and environmental protection. Amsterdam, Elsevier Science, 2002, pp. 69–100.
Wyszkowska J., Wyszkowski M. Effect of cadmium and magnesium on enzymatic activity in soil. Polish J. of Environmental Studies, 2003, vol. 12, no. 4, pp. 473–479.