Changes in the structure of the rhizosphere complexes of actinomycetes of transgenic tomato (Solanum lycopersicum L., Solanaceae, Solanales) with the gene Fe-SOD 1


Tomato transformants (Solanum lycopersicum L.) with the gene of Fe-superoxide dismutase (Fe-SOD 1) from Arabidopsis thaliana L. making the plant resistant to the damaging effect of oxidative stress were used in our work. The original-genotype tomato and independent transgenic lines bn 6 and bn 4 were grown in artificial climate. According to the results of determination of the total SOD activity and lipid peroxidation, line 6 bn differs from both the original genotype and line 4 bn by more balanced lipid homeostasis. Under the influence of gene insertions, realignment occurred in the rhizosphere of the line 6 bn plants, in the structure of their actinomycetes complexes. They were expressed as changes in the occurrence frequency and relative abundance of representatives of individual genera, sections and series in the complex, as well as antagonist species, cellulolytic and auxin producers.


Бабаков А. В., Харченко П. Н. Стрессоустойчивость растений // Проблемы агробиотехнологии / под ред. П. Н. Харченко. М. : ВНИИСХБ, 2012. С. 23 – 48.

Гаузе Г. Ф., Преображенская Т. П., Свешникова М. А., Терехова Л. П., Максимова Т. С. Определитель актиномицетов. Роды Sreptomyces, Streptoverticillium, Chainia. М. : Наука, 1983. 248 с.

Егоров Н. С. Основы учения об антибиотиках. М. : Высш. шк., 1979. 485 с.

Кравченко Л. В., Азарова Т. С., Достанко О. Ю. Влияние корневых экзометаболитов пшеницы с различной плоидностью генома на рост Azospirillum brasilence // Микробиология. 1993. T. 62, № 3. С. 524 – 529.

Лакин Г. Ф. Биометрия : учеб. пособие для биол. спец. вузов. М. : Высш. шк., 1990. 352 с.

Лукаткин А. С. Холодовое повреждение теплолюбивых растений и окислительный стресс. Саранск : Изд-во Морд. гос. ун-та, 2002. 208 с.

Лутова Л. А. Биотехнология высших растений. СПб. : Изд-во СПбГУ, 2010. 238 с.

Определитель бактерий Берджи : в 2 т./ ред. Дж. Хоулт, Н. Криг, П. Снит, Дж. Стейли, С. С. Уилльямс. М. : Мир, 1997. Т. 2. 800 с.

Серенко Е. К., Овчинникова В. Н., Куренина Л. В., Баранова Е. Н., Гулевич А. А., Майсурян А. Н., Харченко П. Н. Получение трансгенных растений томата с геном Fe-зависимой супероксиддисмутазы // Докл. РАСХН. 2009. № 4. С. 12 – 14.

Широких И. Г., Широких А. А., Ашихмина Т. Я. Изучение микробного потенциала агроценозов для повышения продуктивности и стрессоустойчивости растений методами биотехнологии // Научные доклады / Коми науч. центр УрО РАН. Сыктывкар, 2007. Вып. 490. 28 с.

Aira M., Brandón M. G., Lazcano C., Baath E., Domínguez J. Plant genotype strongly modifies the structure and growth of maize rhizosphere microbial communities // Soil Biology and Biochemistry. 2010. Vol. 42, iss. 12. P. 2276 – 2281.

Beauchamp C., Fridovich J. Superoxide Dismutase : Improved Assays and an Assay Applicable to Acrylamide Gels // Analytical Biochemistry. 1971. Vol. 44, № 1. P. 276 – 287.

Bhoomika K., Pyngrope S., Dubey R. S. Differential responses of antioxidant enzymes to aluminum toxicity in two rice (Oryza sativa L.) cultivars with marked presence and elevated activity of Fe SOD and enhanced activities of Mn SOD and catalase in aluminum tolerant cultivar // Plant Growth Regulation. 2013. Vol. 71. P. 235 – 252.

Broeckling C. D., Broz A. K., Bergelson J., Manter D. K., Vivanco J. M. Root exudates regulate soil fungal community composition and diversity // Applied and Environmental Microbiology. 2008. Vol. 74, № 33. P. 738 − 744.

Cartes P., McManus M., Wulff-Zottele C., Leung S., Gutiérrez-Moraga A., Mora M. L. Differential superoxide dismutase expression in ryegrass cultivars in response to short term aluminium stress // Plant Soil. 2012. Vol. 350, № 1 – 2. P. 353 − 363.

Chaudhry V., Dang H. Q., Tran N. Q., Mishra A., Chauhan P. S., Gill S. S., Nautiyal C. S., Tuteja N. Impact of salinity-tolerant MCM6 transgenic tobacco on soil enzymatic activities and the functional diversity of rhizosphere microbial communities // Research in Microbiology. 2012. Vol.163, № 8. P. 511 – 517.

Devos Y., Reheul D., Schrijver A. D. The co-existence between transgenic and non-transgenic maize in the European Union : a focus on pollen flow and cross-fertilization // Environmental Biosafety Research. 2005. Vol. 4, № 2. P. 71 – 87.

Folman L. B., Postma J., Van Veen J. A. Ecophysiological characterization of rhizosphere bacterial communities at different root locations and plant developmental stages of cucumber grown on rockwool // Microbial Ecology. 2001. Vol. 42, № 4. P. 586 – 597.

Gransee A., Wittenmayer L. Qualitative and quantitative analysis of water-soluble root exudates in relation to plant species and development // Plant Nutrition and Soil Science. 2000. Vol. 163, № 4. P. 381 – 385.

Icoz I., Saxena D., Andow D. A., Zwahlen C., Stotzky G. Microbial populations and enzyme activities in soil in situ under transgenic corn expressing Cry proteins from Bacillus thuringiensis // J. Environmental Quality. 2008. Vol. 37, № 2. P. 647 – 662.

Kapur M., Bhatia R., Pandey G., Pandey J., Paul D., Jain R. K. A case study for assessment of microbial community dynamics in genetically modified Bt cotton crop fields // Current Microbiology. 2010. Vol. 61, № 2. P. 118 − 124.

Lee Y. E., Yang S. H., Bae T. W., Kang H. G., Lim P. O., Lee H. Y. Effects of field-grown genetically modified Zoysia grass on bacterial community structure // J. Microbiology and Biotechnology. 2011. Vol. 21, № 4. P. 333 – 340.

Libbert E., Risch H. Interactions between plants and epiphytic bacteria regarding their auxin metabolism // Physiologia Plantarum. 1969. Vol. 22. P. 51 – 58.

Murashige T., Skoog F. A revised medium for rapid growth and bioаssays with tobacco tissue culture // Physiologia Plantarum. 1962. Vol. 15. P. 473 – 497.

Oliveira A. P., Pampulha M. E., Bennett J. P. A two-year field study with transgenic Bacillus thuringiensis maize : effects on soil microorganisms // Science of the Total Environment. 2008. Vol. 405. P. 351 – 357.

Rengel Z. Genetic control of root exudation // Plant and Soil. 2002. Vol. 245, iss. 1. P. 59 – 70.

Tarkka M., Hampp R. Secondary Metabolites of Soil Streptomycetes in Biotic Interactions // Secondary Metabolites in Soil Ecology. Soil Biology 14 / ed. P. Karlovsky. Berlin ; Heidelberg : Springer-Verlag, 2008. P. 107 – 126.

Teather R. M., Wood P. J. Use of Сongo red-polysaccharide interaction in erumeration and characterization of cellulolytic bacteria the bovine rumen // Applied and Environmental Microbiology. 1982. Vol. 43, № 4. P. 777 – 780.

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