Estimation of the Toxicity of a Metal/Carbon Nanocomposite of Copper by Biotesting


The toxic effect of a metal/carbon copper nanocomposite (Me/C Cu NC) on higher plant seeds and the bacterial sensor “Ecolum-8” was evaluated. A significant inhibitory effect of 0.1% nanomaterial on the seedlings of radish seeds was established. Some stimulating effect on the development of the roots of this culture was found at a concen-tration of 1 • 10–8%, but it was insignificant (by 11.3%). When wheat was used as a test object, its root growth was inhibited when the seeds were treated with the nanocompo-site at a concentration of 0.1 and 0.01% (a decrease of 30.2 and 79.6%, respectively). No stimulating effect on the wheat root system was revealed. The preparation also had no effect on the development of the aerial part of wheat seedlings, with the exception of 60.0% growth inhibition in the option of treatment with a 0.1% MeC Cu NC solution. Lyophilized cells of Escherichia coli K12 TG1 strain (pXen7) containing the full lux-operon of Photorhabdus luminescens were used as a second biotesting object. Based on bioluminescence analysis, it was found that all studied concentrations of the copper nanocomposite were highly toxic for the bacterial strain (the toxicity index >70%). The value of the toxicological parameter EC50, corresponding to the concentration of the substance that causes 50% inhibition of the luminescence of the sensory microorganism as compared with the control, turned out to be lower than the minimum tested concen-tration of the MeC NC Cu solutions. The calculation was made mathematically, and the probable value of EC50 was equal to 0.016 μg / ml.


Abramenko N. B. Issledovanie i modelirovanie toksicheskogo dejstviya nanochastic serebra na gidrobiontah [Research and modeling of the toxic effect of silver nanoparticles on hydrobionts]. Diss. Cand. Sci. (Chem). Moscow, 2017. 122 p. (in Russian).
Aleshina E. S., Drozdova E. A. Biotoxicity assessment of carbon nanotubes using biotests based on luminescent microorganisms. Vestnik of the Orenburg State University, 2015, no. 10 (185), pp.126 – 129 (in Russian).
Astafurova T. P., Morgalyov Yu. N., Zotikova A. P., Verhoturova G. S., Mihajlova S. I., Burenina A. A., Zajceva T. A., Postovalova V. M., Cycareva L. K., Borovikova G. V. Effect of nanoparticles of titanium dioxide and aluminum oxide on some morphophysiological characteristics of plants. Tomsk State University J., Boilogical Ser., 2011, vol. 13, no. 1, pp. 113–122 (in Russian).
Gulchenko S. I., Gusev A. A., Zaharova O. V. Prospects for creation antibacterial prepara-tions based on copper nanoparticles. Tambov University Reports, Ser. Natural and Technical Sciences, 2014, vol. 19, iss. 5, pp. 1397–1399 (in Russian).   
Gusev A. A., Zajceva O. N., Polyakova I. A., Gorsheneva E. B., Emel'yanov A. V., Shutova S. V., Romancova S. V., Semiletova S. V., Tkachev A. G., Pilyashenko N. E. Preliminary results of complex biotesting of carbon nanomateral – perspective carrier of medical drug. Tambov University Reports, Ser. Natural and Technical Sciences, 2010, vol. 15, iss. 5, pp. 1538–1540 (in Russian). 
Danilov V., Zarubina A., Eroshnikov G., Solov'eva L. N., Kartashev F. V., Zavil'gel'skij G. B. Sensory bioluminescent systems based on lux operons of various types of luminescent bacteria. Moscow University Biological Sciences Bulletin, 2002, no. 3, pp. 20–24 (in Russian).
Deryabin D. G., Aleshina E. S., Efremova L. V. Application of the inhibition of bacterial bio-luminescence test for assessment of toxicity of carbon-based nanomaterials. Microbiology, 2012, vol. 81, no. 4, pp. 492–497.
Maksimova A. V., Kuznetsova M. V., Demakov V. A. The impact of synthetic nitriles on the morphology and viability of some bacterial species. Biology Bulletin, 2016, vol. 43, no. 6, pp. 547–553. DOI: https://doi.org/10.7868/S0002332916050064
Maslobrod S. N., Mirgorod Yu. A., Borodina V. G., Borshch N. A. Effect of aqueous dispersion systems with silver and copper nanoparticles on seed germination. Elektronnaya obrabotka materialov, 2014, vol. 50, no. 4. pp. 103–112 (in Russian).
Metodicheskie ukazaniya MU 1.2.2635-10Gigiena, toksikologiya, sanitariya. Mediko-biologicheskaya ocenka bezopasnosti nanomaterialov” [Hygiene, toxicology, sanitation. Medical and biological assessment of the safety of nanomaterials]. Moscow, 2010 a. 123 p. (in Russian).
Metodicheskie ukazaniya MU 1.2.2634-10Mikrobiologicheskaya i molekulyarno-geneticheskaya ocenka vozdejstviya nanomaterialov na predstavitelej mikrobiocenoza” [Microbi-ological and molecular genetics assessment of the impact of nanomaterials on representatives of the microbiocenosis]. Moscow, 2010 b. 59 p. (in Russian).
Rahmetova A. A. Izuchenie biologicheskoj aktivnosti nanochastic medi, razlichayushchihsya po dispersnosti i fazovomu sostavu [The study of the biological activity of copper nanoparticles, differing in dispersion and phase composition]. Thesis Diss. Cand. Sci. (Biol). Moscow, 2011. 25 p. (in Russian).
Fedorov A. V., Lekonceva T. G., Zorin D. A., Hudyakova A. V., Trineeva V. V. Efficiency of copper/carbon nanocomposite as a root compound on green cuttings of grape vine (Vitis vinifera L.). Modern Research and Development, 2017, iss. 7 (15), pp. 345– 347 (in Russian).
Ruparelia J. P., Chatterjee A. K., Duttagupta S. P., Mukherji S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomaterialia, 2008, vol. 4, iss. 3, pp. 707–716.
Tamayo L. A., Zapata P. A., Rabagliati F. M., Azócar  M. I., Muñoz  L. A., Zhou X., Thompson G. E., Paez M. A. Antibacterial and non-cytotoxic effect of nanocomposites based in polyethylene and copper nanoparticles. J. Materials Science: Materials in Medicine, 2015, vol. 26, iss. 3, pp. 129. DOI: https://doi.org/10.1007/s10856-015-5475-6

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