Depending on the concentrations of Cu and CuO nanoparticles (NPs) (50, 100 and 500 mg/kg dry matter) introduced into an artificial soil, the influence of Cu NPs upon E. fetida is much stronger than similar CuO doses. In particular, this was reflected in higher mortality (80%) and a 50% weight reduction of the worm (p ≤ 0.05) when the dose of Cu NPs was 500 mg/kg, whereas the validity limit of the test (20%) was not exceeded with a similar dose of CuO. Copper hyperaccumulation in the body of E. fetida under the influence of Cu NPs was noted, while at exposure in a medium with CuO NPs, copper accumulated up to a level of 29 mg/kg with subsequent reduction. As to the activity of antioxidant enzymes, the rates of malondialdehyde , superoxide dismutase decreased and that of catalase increased. The enzymatic activity was quite opposite in the presence of CuO NPs.


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