Preliminary data on the Castor fiber activity influence on the ecosystem biodiversity of small rivers in three steppe clusters within the State Nature Reserve “Volga forest-steppe” were obtained. With zooplankton, amphibians and fish as examples, it was shown that after beaver-made ponds had appeared, the abundance of all these groups increased. In old ponds, the fish abundance decreases whilst the amphibian one continues to increase. In drained ponds, the zooplankton and amphibian abundance decreases while the fishes become more numerous, due to the appearance of better flowability.